Dr. Malik Nawaz Shuja

PhD, PostDoc
Virology and Immunology, Department of Microbiology, 

Kohat University of Science and Technology, Kohat

Email: malik.nawaz@kust.edu.pk & maliknshuja@gmail.com 

https://orcid.org/0000-0002-8255-7675

Mob: +92-300-588-9588

EDUCATION

• PhD. (Virology &Immunology; 2017)

Dissertation- "Development of Antibodies, Infectious Clones and Infectivity Studies of Cotton Leaf Curl Burewala Virus (CLCuBuV)”. (CGPA 3.75/4.0)

PhD Supervisor- Dr. Muhammad Tahir

Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, Pakistan.

Overseas Supervisor- Dr. Stephan Winter

DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany

• Phil in Biotechnology and Genetic Engineering (2008). Institute of Biotechnology & Genetic Engineering, The University of Agriculture, Peshawar, Pakistan (CGPA 3.67/4.0)
• B.Sc (Hons) in Plants Breeding (2005). Plant Breeding & Genetics, The University of Agriculture, Peshawar, Pakistan (CGPA 3.75/4.0)
• HSSC Pre-Medical (2000) (802/1100, 73%) & SSC General Science Group (1998) (680/850, 80%). Cadet College Kohat. BISE-Peshawar, Pakistan

RESEARCH INTEREST

• Current research interests emphasis on the detection and molecular characterization of begomoviral disease epidemics in Pakistan and developing novel technologies for their control. The research ranges from using the sequencing technologies to determine genome and transcriptome data for the begomoviruses, to developing robust, low-cost diagnostic technologies for such plant viruses which appear to be driving the spread of epidemics.
• The development and use of Infectious clones to find the host range and new plant species which serve to be an alternative host of begomoviruses.
• Along with, the focus is on the production of antibodies for the rapid detection of begomoviruses.
• To find out mechanism of Virus-host and Virus-vector interaction

Honors and Awards

• 1^st Prize Winner in the International Conference at IAGSPU Lahore (21-23 May, 2014)
• 6 months Training Award from HEC-IRSIP (May, 2013 To Jan, 2014)
• HEC-Indigenous Fellowship at NUST (2012-2016)
• Ministry of Science & Technology Scholarship in PhD at NUST (2009-2012)
• Four Year Merit Scholarship in B.Sc. (Hons) from University (2001-2005)
• Certificates in Blood Donor Society, Cricket, Hockey, Debates, Anti-Narcotic
• Society, Farabi Science Club etc. (2001-2008)
• Peace Winds Japan Certificate for Excellency (Oct, 2005)

Research Project

• Molecular and biological characterization of begomoviruses infecting cultivated and non-cultivated plants of Khyber Pakhtunkhwa. (0.5 Million)

PROFESSTIONAL TRAINING COURSES AND WORKSHOPS

1.  2021, One day (March 09) workshop on “1st Multi-Disciplinary Student Research Workshop” organized by Kohat University of Science and Technology, Kohat
2.  2019, Two days training (October 30-31) on “Biorisk Management ” organized by  Health Security Partners and Kohat University of Science and Technology, Kohat at KUST
3.  2015 One day (March 31) workshop on “Use of bioinformatics in high throughput sequencing, genomics, proteomics and transcriptomics” organized at National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad
4.  2015 Three days (March 25-27) workshop on “Omics of begomoviruses and its impact on national economy” organized at ASAB, National University of Sciences and Technology (NUST), Islamabad, under USDA-ICARDA project
5.  2014 Two days (March 18-19) training on the “Development and testing of transgenics for Cotton leaf curl virus (CLCuV) disease resistance” organized by CEMB, University of the Punjab, under USDA-ICARDA project
6.  2013 One day (July 08) training on “Introduction to Biosafety” organized by Julius Kuhn Institut (JKI), Deutschland.
7.  2011 “Disability Training Workshop” held on May 19 (resource person- Muhammad Atif, president Special Training Exchange Program), organized by Youth for Peace (YFP).
8.  2011 “First Annual HCV Management Symposium (February 10-11)” held at NUST Center of Virology and Immunology (NCVI), National University of Sciences and Technology (NUST), Islamabad, Pakistan.
9.   2009 Two days national workshop (March 11-12) “Advance Research Techniques and Research Management in Life Sciences” held at NWFP Agricultural University Peshawar under joint higher education links program.
10.  2008 Twelve days training course “Use of Nuclear and Other Techniques in Food and Agricultural Research” (Nov. 3-14), organized by Nuclear Institute for Food and Agriculture (NIFA) Peshawar.
11. 2008 Three days international workshop (May 27-29) “Basic Techniques in Research, Dissertation and Scientific Paper Writing”, organized by Department of Zoology, Kohat University of Sciences & Technology (KUST) in collaboration with HEC Pakistan.
12.   2008 Seven days international thematic workshop on “Biofertilizer Technology” (January 22-28), Organized by COMSTECH and HEC-Pakistan.
13.  2007 Three days’ training course (September 26-28) “Cultivation systems, cultivation techniques and nursery” regarding Olive growing,    organized by Pakistan Oil Seed Development Board (PODB), Peshawar.
14.  2007 Two days’ workshop (July 20-21) “Training of trainers in conservation strategies for plant biodiversity of Pakistan” organized by ‘The Centre of Plant Biodiversity’ University of Peshawar.

PROFESSIONAL EXPERIENCE

STUDENTS SUPERVISED: 

RESEARCH PUBLICATIONS

1. 1. Aamir Lal, Eui-Joon Kil, Thuy Thi Bich Vo, I Gusti Ngurah Prabu Wira Sanjaya, Muhammad Amir Qureshi, Bupi Nattanong, Muhammad Ali, Malik Nawaz Shuja, Sukchan Lee (2022).  Interspecies recombination-led speciation of a novel geminivirus in Pakistan. Viruses, 14, 2166. https:// doi.org/10.3390/v14102166. IF: 4.911
   2. Sanam Banaras, Rehan Zafar Paracha, Maryum Nisar, Ayesha Arif, Jamil Ahmad, Muhammad Tariq Saeed, Zartasha Mustansar, Malik Nawaz Shuja (2022). System Level Modeling and Analysis of TNF-a mediated Sphingolipid Signaling Pathway in Neurological Disorders for the Prediction of Therapeutic Targets, Frontiers in Physiology, section Computational Physiology and Medicine, . 13:872421. doi: 10.3389/fphys.2022.872421. IF: 4.566
   3. Malik Nawaz Shuja, Hasan Riaz, Muhammad Ali, Muhammad Qasim, Muhammad Anees, Stephan Winter, Sabry Hassan , Mohamed M. Hassan and Mohsen Mohamed Elsharkawy (2022). Molecular characterization and identifcation of bhendi yellow vein mosaic virus with satellite DNAs infecting okra plants of district Mardan, Pakistan. Egyptian Journal of Biological Pest Control, 32:49 https://doi.org/10.1186/s41938-022-00550-6 IF: 0.381
   4. Madeeha Mehboob, Muhammad Hakim, Obaid Ullah, Sumaira Salahuddin Lodhi, Irum Khalil, Muhammad Anees, & Malik Nawaz Shuja. (2021). Isolation, Identification and Characterization of Urinary Tract Infectious Bacteria and the Effect of Different Antibiotics. BioScientific Review, 3(3):43-62. https://doi.org/10.32350/BSR.0303.04
   5. Ali, M., Afridi, R. A., Ali, sadiq, Shuja, M. N., & Riaz, H. (2021). Parthenium hysterophorus, an emergent weedy plant species expands its geographical ranges in Pakistan. BioScientific Review, 3(3). https://doi.org/10.32350/BSR.0303.i
   6. Nisa I, Qasim M, Driessen A, Nijland J, Adnan F, Shuja MN, Rahman H (2021) Virulence profiling of Shigella flexneri and emergence of serotype 2b as highly virulent shigellosis causing strain in Pakistan. Infect Genet Evol, 93:104922 IF 3.342
   7. Malik Nawaz Shuja , Hasan Riaz , Muhsin Jamal , Muhammad Imran (2021). Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker? BioScientific Review, 3(1), 01-05. https://doi.org/10.32350/bsr/2021/31/785
   8. Shah, A., Ayub, A., Shuja, M. N., Ali, T., & Akbar, F. (2020). Molecular Characterization of the Begomovirus Associated Satellites Infecting Spinacia Oleracea and Capsicum Annum Plants in Kohat, Pakistan. BioScientific Review, 2(4), 43-56. https://doi.org/10.32350/bsr/2020/24/1334
   9. Aamir Lal, Eui-Joon Kil, Thuy Thi Bich Vo, Chairina Fadhila, Phuong Thi Ho, Malik Nawaz Shuja, Muhammad Ali, and Sukchan Lee (2020). First Report of Duranta leaf curl virus Infecting Ficus virens Showing Leaf Curl Symptoms in Pakistan. Plant Disease Notes; The American Phytopathological Society. https://doi.org/10.1094/PDIS-12-19-2719-PDN
   10. Malik Nawaz Shuja, Muhammad Ali, Taj Ali, Baharullah Khattak, Muhammad Qasim, Stephan Winter (2019). First report of Catharanthus yellow mosaic virus and Pedilanthus leaf curl virus infecting Duranta erecta in Pakistan. Plant Disease Notes; The American Phytopathological Society. 103(5): 1049. https://doi.org/10.1094/PDIS-08-18-1308-PDN
   11. Malik Nawaz Shuja and Muhammad Tahir (2019). Molecular characterization of cotton infecting begomoviruses and DNA satellites associated with Cotton leaf curl disease in Pakistan. EC Microbiology,15(7): 545-560
   12. Inam Ullah, Masood Rabbani, Hameed Ur Rehman, Feroz Khan, Asif Ali, Hazrat Noor, Nasir Ali Khan, Malik Nawaz Shuja, Umer Zeb, Sehrish Khattak, Tahir Ullah, Safiullah Khan Achakzai, Muhammad Rizwan, Mehboob Khan, Abdul Wahab, Abdul Aziz , Nasir Mahmood, Rohi Alam (2019). Bovine viral diarrhea virus and associated risk factors seroprevalence in dairy herds of Khyber Pakhtunkhwa (KP), Pakistan. International Journal of Biosciences, 15(1)): 289-294.
   13. Muhammad Ali Subhani Nasira Wahab , Muhammad Ibrahim, Hameed Ur Rehman, Muhammad Asad, Wajid Ullah, Mohammad Kamil, Muhammad Ikramullah, Muhammad Arif, Muhammad Zubair, Wahid Shah, Malik Nawaz Shuja (2019). Synthesis of silver nanoparticles from plant extracts and their antimicrobial application. International Journal of Biosciences, 14(3): 243-257.
   11. Aftab Ahmad, Fareeha Akhtar, Muhammad Ilyas Khan, Hameed Ur Rehman, Syeda Alia Gerdazi, Uzma Ghani, Muhammad Hanzalah Khan,Uzma Ayaz, Hira Mushtaq, Nausheen Abdul Malik,Nasir Mahmood, Matiullah, Wahid Shah, Malik Nawaz Shuja. (2019). Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat. International Journal of Biosciences. 14 (4): 305-316
   12. Fazal Rahim, Mehboob Khan, Hameed Ur Rehman, Muhammad Ilyas Khan, Hasim Ud Din, Muhammad Zubair, Taimur Khan, Fayaz Khan, Waqas Ahmad, Nasir Mahmood, Khaleel Ullah, Matiullah, Wahid Shah, Malik Nawaz Shuja (2019). Prevalence of Hepatitis B and C virus infection in IDP’s Camp of North Waziristan Agency and FR-Bakakhel of Khyber Pakhtunkhwa, Pakistan. International Journal of Biosciences. 14(3): 237-242
   13. Amir Atlas, Nargis Jamila, Sadiq Noor Khan, Hameed ur Rahman, Ali Nawaz, Hazrat Usman, Matiullah, Malik Nawaz Shuja, Safiullah khan Achakzai, Nasir Mahmood (2019). Incidence of Hepatitis C in different Paramedical Staff Working at Different Hospitals in Pakistan. International Journal of Biology, Pharmacy and Allied Sciences. 8(4): 739-748
   14. Inam Ullah, Nasir Mahmood Khattak, Zahidullah Khan, Hazir Rehman, Hassan Naveed1, Malik Nawaz Shuja, Muhammad Naeem, Matiullah, Ikram Ullah, Hameed Ur Rehman (2019). Isolation, identification and antibiotic susceptibility pattern of Salmonella species isolated from poultry samples of District Kohat. International Journal of Biosciences, 14(1): 284-292.
   15. Feroz Khan, Hameed Ur Rehman, Zahidullah Khan, Asad Ullah, Afzal Ahmad, Malik Nawaz Shuja, Hakim Ullah and Sundas Navid (2018). Malaria is a health problem in pregnant women of Bannu district, Khyber Pakhtunkhwa province, Pakistan. International Journal of Mosquito Research, 5(5): 147-149.
   16. Malik Nawaz Shuja, Hassan Riaz, Muhammad Ali, Irum Khalil, Sidra Anwar, Taj Ali, Muhammad Qasim, (2019). First report of Bhendi yellow vein mosaic virus and cognate Croton yellow vein mosaic betasatellite and Cotton leaf curl Multan alphasatellite in symptomatic Abelmoschus esculentus in Pakistan. Plant Disease Notes; The American Phytopathological Society (Under review)
   17. Muhammad Ali, Sidra Anwar, Malik Nawaz Shuja, Rajiv Kumar Tripathi and Jaswinder Singh (2018). The genus Luteovirus from infection to disease. European Journal of Plant Pathology,151(4): 841-860. DOI:10.1007/s10658-018-1425-8 IF: 1.907
   18. Malik Nawaz Shuja, Muhammad Tahir and Rob W. Briddon, (2017). Occurrence of a recombinant alphasatellite carrying sequences derived from the helper virus in cotton leaf curl disease affected cotton Tropical Plant Pathology. 42(5): 397-402. DOI 10.1007/s40858-017-0161-5 IF 1.488
   19. Malik Nawaz Shuja, Muhammad Tahir (2015). Detection and molecular characterization of cotton infecting begomoviruses from Pakistan. 6^th Congress of European Microbiologists, June 7-11, 2015, Maastricht, The Netherland
   20. Malik Nawaz Shuja, Rob W. Briddon and Muhammad Tahir, (2014). Identification of distinct of Cotton leaf curl Burewala virus (CLCuBuV). Archives of Virology.150 (10)-2787-90. IF 2.574
   21. Malik Nawaz Shuja Wajid Ali, Aqib Iqbal et al., (2011). Maize breeding for marginal lands- Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage. African Journal of. Biotechnology. . 10 (18)-3521-3527.
   22. Malik Nawaz Shuja, Dure Nayab, Muhammad Ali, Aqib Iqbal and Iftikhar Hussain Khalil (2010). Evaluating the response of Wheat Genotypes to Clipping. International. Journal of Agriculture and Biology.12:111-114.
   23. Muhammad Ali, Malik Nawaz Shuja, Muhammad Zahoor and Ishtiaq Qadri (2010). Phytic acid- how far have we come? African Journal of Biotechnology. 9 (11)- 1551-1554

 RESEARCH PUBLICATIONS in PROGRESS

1. Sadia Fazil; Laiba Saeed; Humaira Shah; Fozia Rehman; Muhammad Imran; Hussah M. Alobaid; Ghalia Nawal; Malik Nawaz Shuja; Abid Ali; Nawshad Muhammad; Shah Alam Khan; Vetriselvan Subramaniyan; Talha Bin Emran; Abdur Rahim; Mohammad Nazmul Hasan Maziz; Rusli Bin Nordin; Ikram Shah Bin Ismail (2023). Chitosan and Heparin Based Advanced Hydrogels as Wound Dressings: Their Chemistry, Structure and Biomedical Applications. Journal of Science: Advanced Materials and Devices (Submitted) IF: 5.469
2. Rana Mateen, Young-Jun KIM, Muhammad Arif Nadeem Saqib, Asma Tariq, Aamir Lal, Iqra Naeem, Muhammad Ali, Iqra Sohail Naeem, Malik Shuja, Rukhsana Parveen and Sukchan LeeDrug repurposing: Identification of potential drug targets against nonstructural protein 1 (nsp-1) of SARS-CoV-2 using FDA-approved drugs. Frontiers in Molecular Biosciences, section Biological Modeling and Simulation. (Submitted)
3. Muhammad Ahmad Zeshan, Nadeem Ahmed, Hasan Riaz, Malik Nawaz Shuja (2022), Prediction of tomato leaf curl virus disease and its vector by using multiple regression analysis. Submitted
4. Muhammad Nazir Uddin, Saifullah, Sher Hayat Khan, Musharaf Ahmad, Aqib Iqbal, Wajid Khan, Malik Nawaz Shuja, Mohamed F. Awad and Mohamed M. Hassan (2022), Visualization of interaction between Pochonia Chlamydosporia and Meloidogyne incognita and their impact on tomato. Submitted to Saudi Journal of Biological Sciences
5. Malik Nawaz Shuja, Muhammad Tahir, Sahar Zaidi, (2022). Optimized condition for expression and purification of recombinant coat protein of Cotton leaf curl Kokhran virus – Burewala in Escherichia coli. Protein Expression and Purification (Submitted)
6. Muhammad Ali, Sohail Afzal, Sajjad Khattak, Malik Nawaz Shuja, Hassan Riaz, Mohsin Jamal, Sidra Anwar (2022). Insights into biological and taxonomic features of begomoviruses. Journal of General Virology. (Under Review)
7. Mahbube Alikhani; Seyyede Atefeh Hosseini; Faezeh Sadat Abtahi; Malik Nawaz Shuja (2022). Occurrence and molecular characterization of two potyviruses in Ocimum basilicum in Iran" in Virus Disease (under review)

BOOK CHAPTER

• Zia Rahman, Malik Nawaz Shuja, Muhammad Ali, Sohail Afzal. Chapter 13 Plant Probiotics: Technical Challenges and Emerging Solutions for Enhancing Food Crops. Springer Nature Switzerland AG 2021. DOI.10.1007/978-3-030-73507-4_13

SYMPOSSIUM & CONFERENCES

1. Malik Nawaz Shuja, Muhammad Tahir. Post-resistance breakdown in cotton: Dominancy of a single strain of CLCuV on Indian subcontinent. 8th international geminivirus symposium and the 6th international ssDNA comparative virology workshop, 7–10th November 2016, New Delhi, India pp408
2. Shuja, M.N., Briddon, R.W., Winter, S., Tahir, M. Identification of distinct strain of Cotton leaf curl Burewala Virus. International Conference on “Stress Biology and Biotechnology, Challenges and management “May 2123, 2014. IAGS, University of Punjab, Lahore, Pakistan (Ist Prize Winner)
3. Tahir, M., Shuja, M.N., Briddon, R.W. Identification of distinct strain of Cotton leaf curl Burewala Virus. 3rd International Conference and Exhibition on Metabolomics & Systems Biology March 24-26, 2014 Hilton San Antonio Airport, San Antonio, USA
4. Shuja, Malik Nawaz; Tahir, Muhammad; Winter, Stephan. Molecular characterization, Infectivity and Phylogenetic studies on Begomoviruses infecting cotton in Pakistan. 45. JAHRESTREFFEN DES DPG ARBEITSKREISES “VIRUSKRANKHEITEN DER PFLANZEN“am 14. und 15. Oktober 2013, JKI, Braunschweig, Germany
5. Shuja, MN., Ali, I., Iqbal, A., Munir, I., Sawati, ZA.. Molecular and biochemical markers assisted selection of Maize Lines against drought stress at early growth stages. ISBBE. Aug 3-5, 2009, Shanghai, China
6. Shuja et al. April 20th to 22th, 2010 at the Instituto de Biotecnología de las Plantas (IBP) from Santa Clara, Villa Clara, Cuba
7. International Weed Science Conference Peshawar. Pakistan

MEMBERSHIPS IN SCIENTIFIC SOCIETIES

1. 2018   Member of World Society for Virology (WSV), USA
2. 2017   Member of Society for General Microbiology (SGM), UK
3. 2010   Member of American Society for Microbiology, Washington, USA
4. 2009   Member of Pakistan Botanical Society, Pakistan
5. 2004   Member of Weed Science Society of Pakistan