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Niaz Muhammad (PhD)

Niaz Muhammad (PhD)

Chairman, Department of Medical Laboratory Technology        

Kohat University of Science and Technology Kohat, Pakistan

  • PhD (2011-2015) (Soil Microbiology) Thesis: Impact of Biochar on Microbial Community and Fertility in Acidic Soils Growing Rice, Zhejiang University, Hangzhou, PR China                   
  • M.Phil (2008-2010) (Microbiology) Thesis: Isolation and Screening of Peptidyle Antibiotic(S) Producing Bacteria from Soil: Purification, Characterization and Structure Activity Relationship (SAR), Department of Microbiology Kohat University of Science and Technology Kohat, Pakistan. (GPA 3.97/4.00) 
  • M.Sc (Microbiology), (2004-2005). Federal Urdu University of Arts, Sciences and Technology (73.8%), First division and 2nd position.
  • B.Sc Microbiology (2000-2002) University of Karachi, 2nd division

  • F.Sc Premedical (1997-98), Government College for Men Karachi 1st division,

  • Metric, Science (1995-96), Madrasa Talimul Islam Tablighi College Karachi, Obtained Grade A.


  • Chinese Government Scholarship for PhD in Zhejiang University China from (2011-2015)
  • Merit scholarship for M. Phil from Kohat University of Science and Technology, Kohat, Pakistan.
  • Merit scholarship from federally administered northern areas for M.Sc Microbiology, 2000-2002.
  • Merit scholarship from federally administered northern areas for B.Sc microbiology, 2000-2002.

Work Experience

  • August 2006-27april, research officer in H.E.J, Research institute of Chemistry, University of Karachi
  • April 27, 2007 – Till date, in Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan

Community services Projects completed.

  • Twelve-hundred-liter fixed Biogas plant with zero loss, installation and commissioning in Bagh, AJK (PKR#630,000) in 2023.
  • Twenty-five-hundred-liter fixed Biogas plant with zero loss, installation and commissioning in Bagh, AJK (PKR#680,000) in 2023
  • Twenty-five-hundred-liter fixed Biogas plant with zero loss, installation, and commissioning in Germa, Kohat (PKR#550,000) in 2022
  • Two-thousand-liter fixed Biogas plant with zero loss, installation, and commissioning in village of Hangu, Kohat (PKR#650,000) in 2020
  • Two-thousand-liter fixed Biogas plant with zero loss, installation and commissioning in Bara, Peshawar (PKR#520,000) in 2020
  • Five-hundred-liter Portable Biogas plant with zero loss, installation, and commissioning in Marai Kohat (PKR#380,000) in 2020
  • Pilot scale portable biogas plant designing, installation and commissioning in KDA (PKR#350,000) in 2019
  • First portable Biogas plant was designed in Microbiology laboratory.

Administrative experience

  • Chairman department of MLT
  • Member of scholarship committee KUST
  • Member of University central purchase committee, KUST
  • Member research committee of Microbiology, KUST
  • Academic coordinator, department of Microbiology KUST, 2007- Till date
  • Assistant Warden, City campus Hostel from 2007 to 2011 at Kohat University of Science and Technology, Kohat, Pakistan.

Research students supervised.

M.Phil Scholars Supervised

  1. Warda Kamal (2022) Antibiotic Susceptibility of Bacterial Isolates from Neonatal Sepsis and Inhibition of Drug Resistant Isolates by Selected Medicinal Plant Extracts
  2. Naeem Usman (2022). Studying Diversification of Microbes in Soil Around Industrial Zone oil Fields of District Karak
  3. Mohsin Ali (2022) Control of Root Knot Nemtodes Causing Diseases in Okra Using Antagnostic Plant Growth Promoting Bacteria
  4. Benish jamal (2021) Antibacterial Activity of Medicinal Plants Against Drug Resistant Uropathogens
  5. Saira Shafi (2021) Molecular Characterization of Toxoplasma Gondii and Associated Factors in Pregnant Woman in District Kohat
  6. Sana Raheem (2021) Antibacteria Potential of Kaanchoe Daigremontiana Aloe Barabadensis and Allium Satsativum Against Coliba Cillosis Broiler Checken
  7. Muhammad Farhan(2020) Isolation and Characterization of Pectinase Producing Thermophilic Bacteria from Hot Springs of Chitral
  8. In vitro culturing of Leishmania parasite from different regions of KPK, Pakistan.
  9. Faheem Ullah (2019) Screening of Bacillus Species from Cholistan Desert for Antibiotic Metabolite Production
  10. Faisal Shah (2019) Characterization of Biofilm and Molecular Detection of Ciprofloxacin Resistant Gene Among Clinical Isolates of Salmonella Typhi A
  11. Aleem Ullah (2019) Molecular Characterization and Optimization Of Condition For Thermophilic Lipase Producing Bacteria
  12. Zabehullah (2019). Evaluation of Biochar Impact on Microbial Population and Elemental Concentration Of Red Soil
  13. Irfanullah (2019). Bioremediation of heavy metals by fungi isolated from dust particles obtained from automobile workshops of Peshawar.
  14. Seraj afridi (2019) Molecular characterization and optimized production of sustainable bacterial amylases from springs of islamabad
  15. Robail Farooq (2019) Genotyping of Plasmodium vavex and molecular characterization of antimicrobial resistant gene


BS (Hons) / M.Sc

  • Muhammad Saqib (2020) A Review on The Isolation Of Microbes Causing Spoilage In Lycopersicon Esculantum Mills
  • Mohsin Ali, Faryal Khan (2019) Prevalence and Antimicrobial Susceptibility Pattern of Uro-Pathogens Isolated Patients in Kohat
  • Syed Fatima Gilani (2019) Isolation and Identification of Antibiotic Resistant Bacteria from The Patents of Urinary Tract Infection
  • Muhammad yaseen habibullah (2017) Reduction in aerobic coliforms count during anaerobiosis in biogas plant.
  • Isolation And Inhibition of Fungi Involved Post –Harvest Spoilage of Guava (2016 Mr. Muhammad Fareed Khan and Syed Mir Hassan, Department of Microbiology, KUST)
  • Isolation and identification of soil bacteria producing antibacterial metabolites (2016 Mr. Asifullah, Muhammad Haneef and Muhammad Ibrahim, Department of Microbiology, KUST)
  • Antibacterial activity of medicinal plants (2010, Mr. Qaisar Jamal and Fayaz Ahmed, Department of Microbiology, KUST)
  • Effect of microbial isolates on plant growth (2008, Mr Rashid Azeem, Department of Microbiology, KUST)
  • Microbiological analysis of raw material, products, machinery, environment, wraping material and water of candy land (2007, Mr. Abdul Hameed, Basit Noor and Muhammad Afzal, Department of Microbiology, KUST)

Laboratory Skills

  • Biochar production from organic waste and characterization
  • Microbial isolation, identification, and characterization
  • Fungal isolation, identification, and characterization
  • Production, purification, and characterization of microbial metabolites
  • Microscopy
  • Analysis of Food, Water, and other edible substances
  • Molecular Biology (DNA, RNA extraction, PCR, Real time PCR), Phylogeny
  • Microbial community structure through molecular studies
  • Phylogeny of microbes


 For updated publications, please



  1. Faheem Ullah, Sadir Zaman , N MuhammadEludication of antibacterial Activity of Bacillus sp. And Alcaligenes sp. Metabolites against multidrug-resistant bacteria Nova Biotechnologica et Chimica 3 (DOI: 10.36547/nbc.1337), 1-10
  2. M Afzal, C Tang, M Yu, N Muhammad, H Zhao, J Xu(2022) Water regime is important to determine cadmium toxicity on rice growth and rhizospheric nitrifier communities in contaminated paddy soils Plant and Soil 472 (1-2), 609-628
  3. Muhammad Afzal, Mehreen Saeed, Niaz Muhammad, Baharullah Khattak (2021). Characterization, antibiotic sensitivity assay and phylogenetic analysis of bacterial isolates of river water Journal of Bacteriology & Mycology 9 (1), 7-13
  4. Muhammad Afzal ,Niaz Muhammad, Aamer Ali Shah; (2021), Bacteria isolated from a limestone cave and its ability to produce industrially important enzymes,  Journal of Bacteriology & Mycology, 9, 104-113
  5. Muhammad Afzal, Mehreen Saeed, Niaz Muhammad, 2021, Characterization, antibiotic sensitivity assay and phylogenetic analysis of bacterial isolates of river water Journal of Bacteriology & Mycology, 9, 7-13,
  6. Z Ullah, S Ali, N Muhammad, N Khan, M Rizwan, MD Khan, N Khan (2020), Biochar impact on microbial population and elemental composition of red soil Arabian Journal of Geosciences 13 (16), 1-9
  7. I Khan, Z Noor, S Allauddin Khan, T Ali Khan, N Muhammad(2020), Microscopic and molecular evidence in support of rodent as a reservoir for dissemination of Leishmaniasis Microscopy Research and Technique
  8. Afridi, n ali, m niaz, si hussain, fu farman, bc kim (2020), molecular characterization and production of bacterial amylases from shahdara spring, pakistan, applied ecology and environmental research 18 (2), 2611-2620
  9. Afzal, M., Yu, M., Tang, C., Zhang, L., Muhammad, N., Zhao, H., . . . Xu, J. (2019). The negative impact of cadmium on nitrogen transformation processes in a paddy soil is greater under non-flooding than flooding conditions. Environment international, 129, 451-460.
  10. Akbar, A., Sadiq, M. B., Ali, I., Muhammad, N., Rehman, Z., Khan, M. N., . . . Anal, A. K. (2019). Synthesis and antimicrobial activity of zinc oxide nanoparticles against foodborne pathogens Salmonella typhimurium and Staphylococcus aureus. Biocatalysis and Agricultural Biotechnology, 17, 36-42.
  11. Akbar, A., Sitara, U., Ali, I., Muhammad, N., & Khan, S. A. (2014). Isolation and characterization of biotechnologically potent Micrococcus luteus strain from environment. Pakistan journal of zoology, 46(4).
  12. Akbar, A., Sitara, U., Khan, S. A., Muhammad, N., Khan, M. I., Khan, Y. H., & Kakar, S. U. R. (2013). Drinking water quality and risk of waterborne diseases in the rural mountainous area of Azad Kashmir Pakistan. International Journal of Biosciences, 3(12), 245-251.
  13. Ali Akbar, M. B. S., Imran Ali , Muhammad Anwar , Niaz Muhammad. (2019). Lactococcus lactis subsp. lactis isolated from Fermented Milk Products and Its Antimicrobial Potential. Cyta journal of food, 1-29.
  14. Asad Ullah, M. Q. a., Hazir Rahman, Jafar Khan, Mohammad Haroon, Niaz Muhammad, Abdullah Khan, & Muhammad, N. (2016). High frequency of methicillin‑resistant Staphylococcus aureus in Peshawar Region of Pakistan. springer Plus, 5(1), 1-6.
  15. Baig, S. A., Xu, X., Navedullah, M. N., & Khan, Z. U. (2012). Pakistan’s drinking water and environmental sanitation status in post 2010 flood scenario: humanitarian response and community needs. Journal of Applied Sciences in Environmental Sanitation, 7(1), 49-54.
  16. Baig, S. A., Zhu, J., Muhammad, N., Sheng, T., & Xu, X. (2014). Effect of synthesis methods on magnetic Kans grass biochar for enhanced As (III, V) adsorption from aqueous solutions. Biomass and Bioenergy, 71, 299-310.
  17. Dai, Z., Li, R., Muhammad, N., Brookes, P. C., Wang, H., Liu, X., & Xu, J. (2014). Principle component and hierarchical cluster analysis of soil properties following biochar incorporation. Soil Science Society of America Journal, 78(1), 205-213.
  18. Dai, Z., Meng, J., Muhammad, N., Liu, X., Wang, H., He, Y., . . . Xu, J. (2013). The potential feasibility for soil improvement, based on the properties of biochars pyrolyzed from different feedstocks. Journal of soils and sediments, 13(6), 989-1000.
  19. Dai, Z., Wang, Y., Muhammad, N., Yu, X., Xiao, K., Meng, J., . . . Brookes, P. C. (2014). The effects and mechanisms of soil acidity changes, following incorporation of biochars in three soils differing in initial pH. Soil Science Society of America Journal, 78(5), 1606-1614.
  20. Dai, Z., Zhang, X., Tang, C., Muhammad, N., Wu, J., Brookes, P. C., & Xu, J. (2017). Potential role of biochars in decreasing soil acidification-A critical review. Science of the Total Environment, 581, 601-611.
  21. Hussain, M., Munir, S., Ayaz, S., Khattak, B. U., Khan, T. A., Muhammad, N., . . . Jamal, M. A. (2017). First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan. Asian Pacific Journal of Tropical Medicine, 10(7), 718-721.
  22. Hussain, M., Munir, S., Jalal, A., Khan, T. A., Muhammad, N., Khattak, B. U., . . . Bashir, N. H. (2018). Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan. Asian Pacific Journal of Tropical Medicine, 11(8), 495.
  23. Khan, I., Noor, Z., Allauddin Khan, S., Ali Khan, T., Muhammad, N., Ullah Khattak, B., & Hussain, M. (2020). Microscopic and molecular evidence in support of rodent as a reservoir for dissemination of Leishmaniasis. Microscopy Research and Technique.
  24. Khan, M., Daud, M., Basharat, A., Khan, M. J., Azizullah, A., Muhammad, N., . . . Zhu, S. J. (2016). Alleviation of lead-induced physiological, metabolic, and ultramorphological changes in leaves of upland cotton through glutathione. Environmental Science and Pollution Research, 23(9), 8431-8440.
  25. Mubashir Hussain, S. A., Niaz Muhammad, S. Perveen. (2010). Factors affecting hemagglutination activity of avian influenza viruses (H7N3, H9N2 subtypes. Global Veterinaria, 5(2), 154-157.
  26. Muhammad, N., Akbar, A., Shah, A., Abbas, G., Hussain, M., & Khan, T. (2015). Isolation optimization and characterization of antimicrobial peptide producing bacteria from soil. The Journal of Animal & Plant Sciences, 25(4), 1107-1113.
  27. Muhammad, N., Aziz, R., Brookes, P. C., & Xu, J. (2017). Impact of wheat straw biochar on yield of rice and some properties of Psammaquent and Plinthudult. Journal of soil science and plant nutrition, 17(3), 808-823.
  28. Muhammad, N., Bangush, M., & Khan, T. A. (2012). Microbial contamination in well water of temporary arranged camps: A health risk in northern Pakistan. Water Quality, Exposure and Health, 4(4), 209-215.
  29. Muhammad, N., Brookes, P., & Wu, J. (2016). Addition impact of biochar from different feedstocks on microbial community and available concentrations of elements in a Psammaquent and a Plinthudult. Journal of soil science and plant nutrition, 16(1), 137-153.
  30. Muhammad, N., Dai, Z., Wang, H., Xu, F., & Xu, J. (2013). Impact of pyrolysis time and temperature on physicochemical characteristics of biochars from wetland Plants. In Functions of Natural Organic Matter in Changing Environment (pp. 1039-1044): Springer, Dordrecht.
  31. Muhammad, N., Dai, Z., Xiao, K., Meng, J., Brookes, P. C., Liu, X., . . . Xu, J. (2014). Changes in microbial community structure due to biochars generated from different feedstocks and their relationships with soil chemical properties. Geoderma, 226, 270-278.
  32. Muhammad, N., Hussain, M., Ullah, W., Khan, T. A., Ali, S., Akbar, A., . . . Al-Wabel, M. I. (2018). Biochar for sustainable soil and environment: a comprehensive review. Arabian Journal of Geosciences, 11(23), 731.
  33. Naveedullah Naveedullah1, M. Z. H., Chunna Yu2, Chaofeng Shen1,*, Niaz Muhammad1, Hui Shen1 andYingxu Chen. (2015). Water Quality Characterization of the Siling Reservoir (Zhejiang, China) Using Water Quality Index. CLEAN soil, air, water, DOI: 10.1002/clen.201400126.
  34. Niaz Muhammad, A. A., Rafiq Ullah, Mati Ullah. (2014). Pathogenic Microbial Load Analysis of Ready-to-Eat Meat Products of Namak-Mandi Food Street Peshawar, Pakistan. Journal of Bio-Moleular Sciences (JBMS), 2(1), 21-27.
  35. Niaz Muhammad, N. B., Ali Akbar, Azizullah Azizullah, Mumtaz Khan, Muhammad Qasim, & Rahman, H. (2016). Microbial and toxic metal contamination in well drinking water: potential health risk in selected areas of Kohat, Pakistan. Urban Water Journal(10.1080/1573062X.2016.1173218), 1-7.
  36. Sehrish, A. K., Aziz, R., Hussain, M. M., Rafiq, M. T., Rizwan, M., Muhammad, N., . . . Al-Wabel, M. I. (2019). Effect of poultry litter biochar on chromium (Cr) bioavailability and accumulation in spinach (Spinacia oleracea) grown in Cr-polluted soil. Arabian Journal of Geosciences, 12(2), 57.
  37. Shakir Ullah Khana, , Hazir Rahmana, , 1, , Sultan Ayazb, , Muhammad Qasima, , Abdul Jabbarc, , Mohsin Khurshidd, , Mubashir Hussaina, , Niaz Muhammad , Shoaib Ur Rehmane, , Nawab Alif. (2016). GeneXpert assay for rapid detection of Mycobacterium tuberculosis complex in respiratory specimens from a high TB endemic area of Pakistan. Microbial Pathogenesis.
  38. Taj Ali Khan, H. M., 1 Shamim Saleha,2 Hamid Nawaz Tipu,3 Niaz Muhammad, 1, & Abbas1, M. N. (2016). Interferon-Gamma Improves Macrophages Function against M. tuberculosis in Multidrug-Resistant Tuberculosis Patients. Chemotherapy Research and Practice, 2016(http://dx.doi.org/10.1155/2016/7295390), 6.


Workshops & Conferences Attended

  • Workshop on characterization of Proteins organized by HEJ Research institute of chemistry on January 15-22 2007
  • The National Cadet Corp Certificate of Services, training for 40 days held in January 1997 and1998 in Karachi Government College For Men Nazim abad






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